Part:BBa_K4841008:Design

esaRI70V

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 613
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

None

Source

Amplified from pAC-EsaR using primers that appended the 5′ UTR and a synthetic promoter (apFAB104).

References

1. Shong, J. & Collins, C.H. Engineering the esaR promoter for tunable quorum sensing- dependent gene expression. ACS Synth. Biol. 2, 568–575 (2013).
2. Gupta, A., Reizman, I., Reisch, C. et al. Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit. Nat Biotechnol 35, 273–279 (2017).
3.Shong, J., Huang, Y.-M., Bystroff, C. & Collins, C.H. Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. ACS Chem. Biol. 8, 789–795 (2013).
4.Minogue, T.D., Wehland-von Trebra, M., Bernhard, F. & von Bodman, S.B. The autoregulatory role of EsaR, a quorum-sensing regulator in Pantoea stewartii ssp. stewartii: evidence for a repressor function. Mol. Microbiol. 44, 1625–1635 (2002).